Purdue University researchers have developed a biosensor technology aimed at revolutionizing food safety in agriculture. This innovation stems from a collaboration with the Center for Produce Safety. They sought to create a rapid, user-friendly risk assessment tool for growers of fresh produce, including leafy greens.
Speed and Simplicity in Detection
“Current tests used in the field are slow, lab-based, and also require trained personnel,” explains Mohit Verma, associate professor in the Department of Agricultural and Biological Engineering and one of the researchers on the project. “Our goal was to provide growers with a quick and easy-to-use tool that offers rapid, reliable results.”
The solution lies in biosensors, which detect biological molecules and also provide easily readable outputs. The technology employs Loop-Mediated Isothermal Amplification (LAMP). It is a method similar to PCR but simpler as it operates at a single temperature. This makes it feasible for field use, allowing tests to be conducted in basic settings like water baths.
Field Applications and Benefits
“Our biosensors detect nucleic acids, producing results that can even be quantified using a camera to observe color changes,” Verma elaborates. “By placing this on paper-based devices, we simplify the process further. The user only needs to provide the sample, streamlining the entire testing procedure.”
This technology holds significant implications for food safety, particularly in environments where animal and fresh produce operations are in close proximity. By detecting bacteroides—an order of bacteria indicating fecal contamination—the biosensors can quantify contamination levels, offering insights into the potential risk factors.
Promising Field Tests
Recent field tests have shown promising results. “We collected 100 samples from fields with no nearby animal operations and found almost no contamination,” says Verma. “In contrast, samples taken near animal operations showed high levels of contamination, demonstrating the accuracy and effectiveness of our biosensors.”
Path to Commercialization
The next steps involve simplifying the tests further for broader use. Verma notes, “To make this technology user-friendly and accessible, we’re working on product development and commercialization through my startup company, Krishi, which licenses the technology from Purdue.”
Future Impact on Food Safety
Looking ahead, the broader impact of this research is substantial. By providing growers with proactive tools to identify and manage food safety risks, the biosensors could lead to significant reductions in foodborne contamination, ensuring safer produce.
“With this technology, we can move from a reactive approach to a proactive one in managing food safety,” concludes Verma. “Our goal is to make these biosensors widely available to growers, enhancing food safety across the agricultural industry.”
